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About 400 fish are thought to have been killed by pollution in a river and canal in West Yorkshire.
The Environment Agency is investigating the incident at the River Colne and Broad Canal in Huddersfield.
The dead roach were found in and around the Aspley Basin after the agency received reports of "distressed and dying" fish from local angling groups.
The cause of the pollution is unknown, and officers have appealed to anyone with information to come forward.
Mark West, from the EA, said: "There is no obvious evidence of pollution but we are trying to trace the source.
"It's very unlikely the fish died because of natural causes, such as a lack of oxygen in the water."
Water samples from the river and canal are being tested.
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